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Vinh-Phuc Nguyen

Scientist

About Me

Engineered multiple (8-10) antigen presenting cells (APCs) cell lines to support chimeric antigen receptor (CAR)-modified T cells using viral and non-viral expression systems at Intrexon.
Experience with all processes of tissue culture for cell lines and primary cells: single-cell cloning, PBMC isolation, cell enrichment/depletion.
Experience with human biological samples and infectious agents (BSL2/3 lab).
Expertise in using flow cytometry (FACS) to analyze target or receptor expression, phenotype, activation, proliferation or cytotoxicity, and other assays such as WES, ELISA, DNA/RNA isolation and analysis, qPCR, gene cloning, siRNA/shRNA.
Optimized/Validated bioassays (proliferation, cytotoxicity, cytokine production), and tissue culture conditions (growth, expansion, cryopreservation) resulting in process or downstream assay improvement.
Excellent track record in GLP/GMP environment: 1) Achieved a >95% on-time, accurate data delivery in performing GLP/GMP assays, 2) Increased revenue by 45% and testing efficiency by 15% by validating new qPCR assays
Excellent record of staff management: Trained 5 new lab operators (rate of <5% error in performing GLP/GMP assays).
Managed team of 8-10 lab operators (BioReliance), 2 lab technicians and 3 students (UCI and NIH).
Authored 8-10 SOPs.
Resolved more than 40 protocol deviations.
Created key databases (primers, probes, standards) to ensure testing continuity.
Proficiency with different softwares: FACSDiva, FlowJo, LIMS, SDS, SoftMax, GraphPad, Geneious, etc.
Detail-oriented, excellent time management and interpersonal skills to complete multiple simultaneous projects in cross-functional teams

Work Experience

Assay Development- Intrexon
Scientist
November, 2015 - Present
  • Responsibilities and Achievements: 1. Accelerate completion of CAR-T projects by engineering cell lines to support modified CAR T cells: In 9 months, created 8-10 new antigen presenting cell (APC) lines and 3 target lines to expand various CAR T cells 2. Establish SOPs for various assays: co-authored 3-4 SOPs and participated in Electronic Lab Notebook (ELN) launch for recordkeeping, and conducted multiple optimization assays for validation 3. Create inventory databases (antibody, cell bank) to improve lab work efficiency and management


BioReliance
, Scientist
April, 2014 - October, 2015
  • Responsibilities and Achievements: 1. Increased lab revenue by 45% and testing efficiency by 15% by validating new qPCR assays (Pinnacle Mycoplasma and Human Panel) 2. Authored 8-10 new SOPs.
  • Resolved more than 40 protocol deviations.
  • Identified root causes and implemented corrective actions (CAPAs) 3. Trained 5 new research associates to perform GLP/GMP assays with a <5% error rate in testing 4. Managed team of 8-10 research associates to perform GLP/GMP assays 5. Created and maintained 3 key inventory databases of critical lab reagents (primers, probes, positive controls) using LIMS and Excel spreadsheets

BioReliance
, Associate Scientist III
October, 2013 - April, 2014
  • Responsibilities and Duties: 1. Achieved a >95% on-time accurate data delivery in GLP/GMP assay testing 2. Validated new qPCR assay (Pinnacle Mycoplasma assay) to generate ~15% lab revenue 3. Analyzed data and maintained data records for study integrity and archive

NIH
, Special Volunteer Fellow
October, 2012 - July, 2013
  • 1 project - 1 publication Project: Uncover the functions of a new subdomain in the TRIF amino-terminus protein that modulates the protein stability and signaling in response of pathogen infection Achievement: 1. Revealed the functions of a novel subdomain to be crucial for TRAF3 interaction, and involved in TRIF stability 2. Demonstrated this novel subdomain was involved interferon signaling, thereby important for cell immunity against pathogens
  • 1 project - 1 publication Project: Uncover the functions of a new subdomain in the TRIF amino-terminus protein that modulates the protein stability and signaling in response of pathogen infection Achievement: 1. Revealed the functions of a novel subdomain to be crucial for TRAF3 interaction, and involved in TRIF stability 2. Demonstrated this novel subdomain was involved interferon signaling, thereby important for cell immunity against pathogens

NIH
, Senior Research Fellow
September, 2003 - August, 2011
  • 2 Projects - Managed 1 junior post-doc and 1 lab tech - 3 publications Project 1. Investigate the mechanisms of action of IL-9 in leukemia development in ATL pathogenesis Achievement: 1. Designed/Implemented cytokine screening assays 2. Identified IL-9 in the screening/Established that IL-9 promotes a paracrine growth feedback for survival and proliferation of leukemic cells Project 2. Elucidate the roles of LIF in monocytes in ATL pathogenesis Achievement: 1. Demonstrated that the LIF receptor is expressed on the monocytes involved in ATL development 2. Created a database of gene expression profile of LIF-stimulated monocytes 3. Screened this database for potential biomarkers for prognostic/diagnostic of leukemic patients

University of California, Irvine
, Postdoctoral Fellow
July, 1998 - August, 2003
  • 2 Projects - Trained 2 techs and 3 undergraduate students - 2 publications Project 1. Investigate the functions of Stat2-binding domain of the interferon-alpha receptor subunit 2 (IFNaR2) Achievement: 1. Established that the Stat2 “pre-docking” site on the IFNaR2 subunit was crucial for interferon-alpha signaling Project 2. Characterize the contribution of Suppressor of Cytokine Signaling-1 (SOCS-1) and SOCS-3 proteins as negative regulators of IFN-alpha signaling Achievements: 1. Spearheaded this project by generating a database of SOCS cDNAs and stable SOCS-expressing cell lines 2. Identified SOCS-1 and -3 proteins as key inhibitors of interferon-alpha signaling/Elucidated their mechanisms of action
  • 2 Projects - Trained 2 techs and 3 undergraduate students - 2 publications Project 1. Investigate the functions of Stat2-binding domain of the interferon-alpha receptor subunit 2 (IFNaR2) Achievement: 1. Established that the Stat2 "pre-docking" site on the IFNaR2 subunit was crucial for interferon-alpha signaling Project 2. Characterize the contribution of Suppressor of Cytokine Signaling-1 (SOCS-1) and SOCS-3 proteins as negative regulators of IFN-alpha signaling Achievements: 1. Spearheaded this project by generating a database of SOCS cDNAs and stable SOCS-expressing cell lines 2. Identified SOCS-1 and -3 proteins as key inhibitors of interferon-alpha signaling/Elucidated their mechanisms of action

Baylor College of Medicine
September, 1993 - June, 1998
  • 1 Thesis Project - 2 publications Characterize the molecular interactions between viral glycoproteins leading to coronavirus formation

Baylor College of Medicine
, Ph.D.
September, 1993 - June, 1998
  • 1 Thesis Project - 2 publications Characterize the molecular interactions between viral glycoproteins leading to coronavirus formation 1. Established a coexpression system to produce coronavirus-like particles (VLPs) 2. Demonstrated the molecular requirements for incorporation of the spike (S) glycoprotein in VLPs

Education

Baylor College of Medicine, Ph.D.
1993 - 1998
Baylor College of Medicine, Ph.D. - Molecular Virology
1993 - 1998
University of Montreal, B.S. - Microbiology&Immunology
1988 - 1991

Skills

  • Biotechnology, GLP, GMP, Molecular Biology, Immunology, gene cloning, ELISA, flow cytometry, antibody characterization, recombinant protein expression and purification, cell proliferation, immunofluorescence
  • DATABASE
  • GENE EXPRESSION
  • IFN
  • INFORMATION FUZZY NETWORKS
  • SOCS
  • VIROLOGY
  • DATABASES
  • INVENTORY
  • SOPS
  • GLP
  • GMP
  • MYCOPLASMA
  • QPCR
  • TESTING
  • EXCEL
  • LIMS
  • ANTIBODY
  • OPTIMIZATION
  • BIOTECHNOLOGY
  • CGMP
  • ASSOCIATE
  • BACTERIAL
  • BIOASSAYS
  • CELL CULTURE
  • CELL LINE
  • CLONING
  • CYTOTOXICITY
  • DETAIL-ORIENTED
  • DNA
  • DNA/RNA
  • ELISA
  • ENGINEER
  • EXCELLENT MULTITASKER
  • FERMENTATION
  • FLOW CYTOMETRY
  • IMMUNOLOGY
  • MOLECULAR BIOLOGY
  • PROTEIN ANALYSIS
  • PURIFICATION
  • RNA
  • SIMULTANEOUS
  • TIME MANAGEMENT
  • TISSUE CULTURE
  • TRAINING

Languages

Vietnamese
Native or bilingual proficiency
French
Full professional proficiency
English
Full professional proficiency

Contact

Rockville, MD

Networks